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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(11): 724–728
CN 31-1940/Q
Dynamics of Primary Events in the Photocycle of Excited
Bacteriorhodopsin
Jun-Jun LU, Ming MING1, Yi YANG, Jia WU1, Bo
LI, Jian-Dong DING1, Qing-Guo LI2, and Shi-Xiong QIAN*
Department of
Physics, Fudan University, Shanghai 200433, China;
1Department of Macromolecular Science, Key
Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai
200433, China;
2Department of Physiology and Biophysics,
School of Life Sciences, Fudan University, Shanghai 200433, China
Abstract Transient dynamic
behavior of the
excited bacteriorhodopsin (BR), which was isolated from the strain H.
salinarum, was studied at excitation wavelength from 585 to 639 nm. With
the one-color femtosecond (fs) pump-probe technique, we revealed the primary
events in BR�s photocycle
that took place in an ultrafast time scale. From the analysis of the decay components of the
dynamical traces, it was evident that the isomerization of the retinal
chromophore in BR and the intermediate J�s formation occurred
within 500 fs. BR exhibited pH-dependent dynamical behaviors. When the medium
pH was between 5 and 9, the BR ultrafast dynamics has no obvious change. In
contrast, the dynamical curves were obviously affected when the pH was out of
that region.
Key words bacteriorhodopsin; transient dynamics; primary events; femtosecond
laser; pump-probe technique
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Received: July 5, 2004 Accepted: September 29,
2004
This work was supported by the grants from the National Natural
Science Foundation of China (No. 10274103, No. 20174006, No. 20221402, and No.
20374015)
*Corresponding author: Tel, 86-21-65642084; Fax, 86-21-65641344;
Email, [email protected]