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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(11): 741–748
CN 31-1940/Q
Shock Waves Increase T-cell Proliferation or IL-2 Expression by
Activating p38 MAP Kinase
Tie-Cheng YU*, Yi
LIU, Yan TAN1, Yanfang JIANG1, Xueqing ZHENG2,
and Xinxiang XU
Department of Orthopedics, 1Central Laboratory, and 2Lithotriptic Center, the First Teaching Hospital of Jilin
University, Changchun 130021, China
Abstract Shock waves were elicited by transient pressure disturbances, which could
be used to treat musculoskeletal disorders. In present studies, we investigated
whether the low-density shock waves (LDSWs), which are able to damage plasma
membrane without impairing the vimentin or other organelles, might augment
T-cell proliferation as well as IL-2 expression, and if mitogen activated
protein kinase p38 (p38 MAPK) might be an underlying mechanism
through which the LDSWs enhanced T-cell function. We found that the LDSWs
increased activation of p38 MAPK in Jurkat T cells. The LDSWs alone didn�t result in the T-cell proliferation
and IL-2 expression. However, in combination with other stimuli, LDSWs could
augment the T-cell proliferation
and IL-2 expression. Inhibition of p38 MAPK using SB203580 reduced the
stimulatory effects of the LDSWs, which indicated that the LDSWs enhanced IL-2
expression through a mechanism that involved p38 MAPK activation. We concluded
that the p38 MAPK activation played a key role in the regulation of T cell
function by the LDSWs.
Key words shock waves; p38 MAP kinase; IL-2; T-cell proliferation
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Received: July 23, 2004 Accepted: September 17,
2004
*Corresponding author: Tel, 81-431-7930754; E-mail, [email protected]