https://www.abbs.info
E-mail: [email protected]
ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(11): 773–779
CN 31-1940/Q
Accumulation of Pathogenesis-related Type-5 Like Proteins in
Phytoplasma-infected Garland Chrysanthemum Chrysanthemum coronarium
Bo-Xiong ZHONG* and Yan-Wei
SHEN
Department of
Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou
310029, China
Abstract Soluble proteins
extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum
plants infected by onion yellows phytoplasma were analyzed by two-dimensional
gel electrophoresis. Computerized matching analysis revealed that at least six
soluble proteins were accumulated specifically in phytoplasma-infected garland
chrysanthemum. N-terminal amino acids sequences of these soluble proteins,
determined by Edman degradation, shared high sequence similarities with those
of pathogenesis-related type-5 (PR-5) proteins such as tobacco thaumatin-like
protein. Accumulation of these six proteins was also found in garland
chrysanthemum plants infected by other phytoplasmas. These results demonstrate
that phytoplasmal infection induces the accumulation of PR-5 like proteins in
garland chrysanthemum plants.
Key words phytoplasma; garland
chrysanthemum Chrysanthemum coronarium; pathogenesis-related proteins;
thaumatin; osmotin
—————–
Received: August 6, 2004 Accepted: September 27,
2004
*Corresponding author: Tel, 86-571-86971302; Fax, 86-571-86945417;
E-mail, [email protected]