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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(11): 780–785
CN 31-1940/Q
Isolation and Characterization of Photosystem II of Porphyra
yezoensis Ueda
Zheng-Quan GAO1,2, Guang-Ce
WANG1*, and Cheng-Kui TSENG1
1Key
Laboratory of Experimental Marine Biology, Institute of Oceanology, the Chinese
Academy of Sciences, Qingdao 266071, China;
2Graduate
school, the Chinese Academy of Sciences, Beijing 100039, China
Abstract The thylakoid membranes were
isolated and purified from gametophyte of Porphyra yezoensis Ueda (P.
yezoensis) by sucrose density gradient ultracentrifugation. After P.
yezoensis gametophyte thylakoid membranes were solubilized with SDS, the
photosystem II (PSII) particles were isolated and purified. The activity of
PSII particles was determined with DCIP (2,6-dichloroindophenol) photoreduction
reaction. The composition of purified PSII particles was detected by SDS-PAGE.
As a result, seven proteins including 55 kD protein, 47 kD protein, 43 kD
protein, 33 kD protein, 31 kD protein, 29 kD protein, and 18 kD protein were
found. Compared with PSII particles of higher plants and other algae, they were
identified as D1/D2 complex, CP47, CP43, 33 kD protein, D1, D2 and cyt c-550
respectively. Besides, other three new proteins of 20 kD, 16 kD and 14 kD
respectively were found. Among these extrinsic proteins, the 16 kD and 14 kD
proteins had not been reported previously, and the 20 kD protein was found for
the first time in multicellular red algae.
Key words red algae;
Porphyra yezoensis Ueda; photosystem II; polypeptide composition; extrinsic
protein
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Received: July 20, 2004 Accepted: October 8,
2004
This work was supported by the grants from the National Natural
Science Foundation of China (No. 30170499, No. 40476059, No. 30250003, and No.
39890390) and the Project of Scientific Innovation of Institute of Oceanology,
the Chinese academy of Sciences (2002-2005)
*Corresponding author: Tel, 86-0532-2898575; Fax, 86-0532-2880645;
E-mail, [email protected]