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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(12): 793–797
CN 31-1940/Q
Changes in Autoantibodies against b1-Adrenoceptor and M2-Muscarinic Receptor during Development of Renovascular Hypertension
in Rats
Jian-Ming ZHI*, Rong-Fang CHEN1, Heng WU2,
Hui-Rong LIU2, and Rong-Rui ZHAO2
Department of
Physiology, Shanghai Second Medical University, Shanghai 200025, China;
1Department of Toxicology, Second Military
Medical University, Shanghai 200433, China;
2Department of Physiology, Shanxi Medical
University, Taiyuan 030001, China
Abstract In an experimental rat‘s renovascular hypertension model, we studied the genesis of anti-cardiac
b1-adrenoceptor and M2-muscarinic receptor autoantibodies
in relation to the changes in immunological function during the development
of renal hypertension. The biological activities of these autoantibodies were
also examined. It was shown that after two weeks of operation both the frequency
of occurrence and the titre of autoantibodies to cardiac b1-adrenoceptor and M2-muscarinic receptor were significantly
increased as compared with the control of pre-treatment. The increased autoantibodies
lasted for several weeks and then automatically decreased gradually to the
pre-clipping level at 10 weeks. Meanwhile the ratio of CD4+/CD8+ was also undergone an initial increase followed by gradual recovery
and correlated well with the changes in antibody titre. The biological effects
of these autoantibodies displayed an agonistic-like activities on the beating frequency of cultured neonatal
cardiomyocyte. It is suggested that autoimmune mechanisms are involved in
the pathogenesis of renal hypertension and the cardiac receptor autoantibodies
might be one of the mechanisms leading to cardiac dysfunction.
Key words autoantibody; renal hypertension; cardiac receptor; immunology
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Received: June 9, 2004 Accepted: October 2,
2004
*Corresponding author: Tel, 86-21-63846590-776446; E-mail, [email protected]