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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(12): 811–816
CN 31-1940/Q
Identification and Characterization of GH Receptor and Serum GH-binding
Protein in Chinese Sturgeon (Acipenser sinensis)
Zhi-Yong LIAO1,2 and Shang-Quan ZHU2*
1College of Life Sciences, Jiangxi Normal
University, Nanchang 330027, China;
2Key Laboratory of Proteomics Biochemistry and
Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of
Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031,
China
Abstract Chinese sturgeon, a kind
of cartilage ganoid, has a history of over one billion years and it is called
the living fossil of aquatic biology since it keeps some evolutionary trace.
Here, we characterized the growth hormone receptor (GHR) and serum growth
hormone binding protein (GHBP) of Chinese sturgeon. It was shown that GHR was
expressed in various tissues, mainly in hepatic, kidney and intestine tissues.
GHR on the hepatic membrane has high and specific affinity for bream GH (brGH)
and Scatchard analysis of the binding data showed a single class of high
affinity binding site with an association constant Ka of 3.1109 M1. A specific band around
94 kD was detected by SDS-PAGE in cross-linking studies of membrane receptors.
After incubation of Chinese sturgeon serum with 125I-brGH,
a 125I-brGH-GHBP complex was identified by Sephadex G-75, indicating that
in the serum exists GHBP specially binding to brGH.
Key words Chinese sturgeon; growth
hormone receptor; growth hormone binding protein; ligand binding
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Received: September 3, 2004 Accepted: October 28,
2004
This work was supported by a grant from the National and Natural
Science Foundation of China (No. 30460036)
*Corresponding author: Tel, 86-21-54921283; Fax 86-21-54921011;
E-mail, [email protected]