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ISSN 0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(2):93-98 CN 31-1300/Q
Molecular Cloning
of TSARG6 Gene Related to Apoptosis in Human Spermatogenic Cells
Gang LIU, Guang-Xiu LU*, and Xiao-Wei XING
( Human Reproductive and Stem cell Engineering Institute, Central South University,
Changsha 410078, China )
Abstract Beginning from a
mouse EST (GenBank accession No. BE644537) which was significantly up-regulated
in cryptorchidism and represented a novel gene, we cloned a new gene (GenBank
accession No. AY138810) which is related to apoptosis in human spermatogenic
cells by means of GeneScan program and PCR technology. The gene whose full cDNA
length is 1875 bp containing 8 exons and 7 introns is located in human chromosome
11q13.3. Its protein containing 316 amino acid residues is a new member of HSP40
protein family because the sequence contains the highly conserved J domain which
is present in all DnaJ-like proteins and is considered to have a critical role
in DnaJ-DnaK protein-protein interactions. TSARG6 protein displays a 45% identity
in a 348-amino acid overlap with DJB5_HUMAN protein. The result of RT-PCR and
Northern blot analysis showed that TSARG6 is specifically expressed in adult
testis and the transcript is 1.8 kb. Based upon all these observations, it is
considered that we cloned a new gene which probably inhibited human testis spermatogenesis
apoptosis.
Key words gene cloning; PCR;
GeneScan; cryptorchid testis; heat shock protein (HSP); apoptosis
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Received: September 22, 2003 Accepted: November 22, 2003
This work was supported by a grant from the Major State Basic Research Development
of China (No. G1999055901)
*Corresponding author: Tel, 86-731-4373187; Fax, 86-731-4497661; E-mail, [email protected]
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