https://www.abbs.info
E-mail:[email protected]
ISSN 0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(2):123-127 CN 31-1300/Q
The Inhibitory
Role of Lactacystin and β-actacystin
on T-cell Activation and Proliferation
Peng-Hong SONG*,
Hai-Yang XIE, Shu-Sen ZHENG, and Jian WU
( The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou
310003, China )
Abstract To evaluate the
effects of proteasome inhibitors lactacystin (LAC) and β-lactacystin (β-LAC)
on the proliferation and activation of T lymphocytes, flow cytometry was used
to analyze the proliferation and the expression of CD69, CD25 and CD3 of T lymphocytes
activated by PHA. Furthermore, the expressions of PA28 and IL-2 mRNA were assayed
by competitive RT-PCR. The results indicated that: (1) LAC and β-LAC significantly
decreased the incorporation of BrdU and inhibited T lymphocytes proliferation
in T lymphocytes activated by PHA; (2) although LAC and β-LAC did not affect
the expression of CD69 at any time, they significantly inhibited the expression
of CD25 (48 h, 72 h, P<0.05); (3) in comparison with control, LAC
and β--LAC significantly down-regulated the expression of PA28 and IL-2 mRNA
(48 h, 72 h, P<0.05). LAC and β-LAC significantly inhibited the proliferation
and activation of T cells. Mechanisms involved are inhibition of CD25 and down-regulation
of PA28 and IL-2 mRNA expressions.
Key words proteasome; T cells
activation; immunosuppression
—————–
Received: October 20, 2003 Accepted: December 8, 2003
This work was supported by a grant from the Doctoral Program Foundation of Education
Ministry
*Corresponding author: Tel, 86-571-87236570; Fax, 86-571-87236570; E-mail, [email protected]