https://www.abbs.info
E-mail:[email protected]
ISSN 0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(4):297-332 CN 31-1300/Q
Purification
of a Novel Antibacterial Short Peptide in Earthworm Eisenia foetida
Yan-Qin LIU1,2, Zhen-Jun
SUN1*, Chong WANG1, Shi-Jie LI2, and Yu-Zhi LIU2
( 1College of Resource and Environment Science, China Agricultural University,
Beijing 100094, China;
2College of Animal Science, Hebei Agricultural University, Baoding 071001, China
)
Abstract A novel antimicrobial
short peptide was purified from earthworm (Eisenia foetida) by a five-step
protocol including ammonium sulfate precipitation, ultrafiltration, DE-52 ion
exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase
HPLC techniques. The purified peptide was applied to the MALDI-TOF MS to determine
the molecular mass and was also subjected to TOF MS-MS analysis to determine
the amino acid sequence. As a result, a novel antibacterial peptide, named OEP3121,
was obtained, with the molecular mass of 510.8 Da and the sequence being “ACSAG”.
Key words antibacterial peptide;
earthworm; purification
—————–
Received: November 24, 2003 Accepted:
February 12, 2004
This work was supported by the grants from the National Natural Science Foundation
of China (No. 30270195) and the Doctorial Foundation of National Education Committee
of China (20020019029)
*Corresponding author: Tel, 86-10-62892942; Fax, 86-10-62892498; Email, [email protected]