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ISSN 0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(4):309-313 CN 31-1300/Q
Expression
of a Modified Cry1Ie Gene in E. coli and in Transgenic Tobacco
Confers Resistance to Corn Borer
Yun-Jun LIU, Fu-Ping
SONG1, Kang-Lai HE1, Yuan YUAN, Xiao-Xia ZHANG, Peng GAO,
Jian-Hua WANG, and Guo-Ying WANG *
( State Key Laboratory for Agrobiotechnology, National Center for Maize Improvement,
China Agricultural University, Beijing 100094, China;
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute
of Plant Protection, CAAS, Beijing 100094, China )
Abstract The
wild-type Cry1Ie gene from Bacillus thuringiensis was modified
for its efficient expression in transgenic plants. Modified Cry1Ie gene
(designated as Cry1Iem) was cloned into prokaryotic expression vector
pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays
using crude expression products in E. coli revealed that Cry1Iem protein
had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene
was then inserted downstream of the maize ubiquitin-1 promoter in plant expression
vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal
activity against corn borer.
Key words Bacillus thuringiensis;
Cry1Iem; transgenic tobacco; insect-resistance
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Received: December 29, 2003 Accepted:
February 26, 2004
This work was supported by the grants from the High Technology Research and
Development Program of China (2001AA212051) and State Plant
Transformation and Industrialization Program (JY03-B-15)
*Corresponding author: Tel, 86-10-62892577; Fax, 86-10-62892012; E-mail, [email protected]