https://www.abbs.info E-mail: [email protected]
ISSN
0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(6): 405-411 CN 31-1300/Q
Transcriptional Regulation of Urokinase Receptor in High- (95D) and Low-metastatic
(95C) Human Lung Cancer Cells
Chun-Yang LI, Li TAN, Guo-Jing ZHANG, Ping LI, Chang TONG, Jing FAN,
and Yun-Song ZHU*
Key Laboratory, the Education Ministry,
Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan
University, Shanghai 200032, China
Abstract To study the transcriptional
regulation of urokinase receptor (uPAR) in high- (95D) and low-metastatic (95C)
human lung cancer cells, we performed PCR to amplify 2238 bp uPAR promoter from
95C and 95D cells. According to the results of sequencing, five different bases
are found in uPAR promoter between 95C and 95D cells. The results of luciferase
activity assay showed that these differences have no significant effect on the
uPAR promoter activity. Based on a normal uPAR promoter, progressive truncated
mutants were constructed. The transient transfection/reporter assay showed that
the promoter region from –136 to +9 may interact with relevant nuclear factors,
which result in different levels of uPAR expression between 95C and 95D cells.
Key words uPAR; promoter;
transcriptional regulation
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Received: March 18, 2004 Accepted: May 9, 2004
The work was supported by a grant from the National Natural Science Foundation
of China (No. 30170398)
*Corresponding author: Tel, 86-21-54237278; E-mail,
[email protected]