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ISSN 0582-9879 Acta Biochimica et Biophysica Sinica 2004, 36(7): 508–512 CN 31-1300/Q
The Theaflavin Monomers Inhibit the Cancer Cells Growth in Vitro
You-Ying TU1,3, An-Bin TANG2, and Naoharu WATANABE3*
1Department of Tea Science,
Zhejiang University, Hangzhou 310029, China; 2Sichuan Dongfang Insulation Material Co.,
Ltd., Mianyang 621000, China; 3Department of Applied Biological Chemistry, Shizuoka University, 836
Ohya, Shizuoka 422-8529, Japan
Abstract The inhibition effects
of tea theaflavins complex (TFs), theaflavin-3-3‘-digallate (TFDG),
theaflavin-3‘-gallate (TF2B), and an unidentified compound (UC) on the
growth of human liver cancer BEL-7402 cells, gastric cancer MKN-28 cells and
acute promyelocytic leukemia LH-60 cells were investigated. TFs was obtained
through the catalysis of catechins with immobilized polyphenols oxidase. TFDG,
TF2B and UC were isolated from TFs with high speed countercurrent
chromatography (HSCCC). The results showed that TF2B significantly inhibited
the growth of all three kinds of cancer cells, TFs, TFDG and UC had some effect
on BEL-7402 and MKN-28, but little activity on LH-60. The inhibition effects of
TF2B, TFDG, and UC on BEL-7402 and MKN-28 were stronger than TFs. The
relationship coefficients between monomer concentration and its inhibition rate
against MKN-28 and BEL-7402 were 0.87 and 0.98 for TF2B, 0.96 and 0.98 for UC,
respectively. The IC50 values of TFs, TF2B, and TFDG were 0.18,
0.11, and 0.16 mM on BEL-7402 cells, and 1.11, 0.22, and 0.25 mM on MKN-28
cells respectively.
Key words theaflavin monomer;
separation; cancer cells; inhibition
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Received: April 1, 2004 Accepted: May 12, 2004
*Corresponding author: Tel/Fax, 81-54-2384870; E-mail,
[email protected]