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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(8): 553–558
CN 31-1940/Q
Signal Peptide of Potato PinII Enhances the
Expression of Cry1Ac in Transgenic Tobacco
Yun-Jun LIU, Yuan YUAN, Jun
ZHENG, Ya-Zhong TAO, Zhi-Gang DONG, Jian-Hua WANG, and Guo-Ying WANG*
State Key Laboratory for Agrobiotechnology and National Center for
Maize Improvement, China Agricultural University, Beijing 100094, China
Abstract The modified Cry1Ac was
expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein
into the intercellular space, the signal peptide sequence of potato proteinase
inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region.
Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The
results showed that pinII signal peptide sequence enhanced the expression of
Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic
tobacco plants. GFP gene was also fused to the signal peptide sequence and
transformed to tobacco. The results of fluorescent detection showed that GFP
had localized in the apoplast of transgenic plants.
Key words Cry1Ac; signal peptide;
GFP; transgenic tobacco
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Received: March 15, 2004 Accepted: July 2, 2004
This work was supported by the
grants from the High Technology Research and Development Program of China (No.
2001AA212051) and State Plant Transformation and Industrialization Program
(JY03-B-15)
*Corresponding author: Tel,
86-10-62892577; Fax, 86-10-62892012; E-mail, [email protected]