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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(9): 618–622
CN 31-1940/Q
Trichostatin A Extends the Lifespan of Drosophila melanogaster by
Elevating hsp22 Expression
Dan TAO#, Jun LU#, Hui SUN, Yan-Mei ZHAO, Zhi-Gen
YUAN, Xiao-Xue LI, and Bai-Qu HUANG*
Institute of Genetics and Cytology, Northeast Normal University,
Changchun 130024, China
Abstract The level of acetylation
of histones in nucleosomes is related to the longevity of yeast and animals. However,
the mechanisms by which acetylation and deacetylation affect longevity remain
unclear. In present study, we investigated the influence of histone acetylation
modification on the expression of hsp22 gene and the lifespan in Drosophila
melanogaster using histone deacetylase (HDAC) inhibitor Trichostatin A
(TSA). The results showed that TSA could extend the lifespan of Drosophila
melanogaster. Furthermore, TSA significantly promoted the hsp22 gene
transcription, and affected the chromatin morphology at the locus of hsp22
gene along the polytene chromosome. Present data implicate that TSA may affect
the lifespan of Drosophila through changing the level of histone
acetylation and influencing the expression of hsp22 gene that is related
to aging.
Key words histone acetylation;
histone deacetylase inhibitor; Drosophila melanogaster; hsp22;
lifespan
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Received: Match 26, 2004 Accepted: July 2, 2004
This work was supported by the grants from the National Natural
Science Foundation of China (30370316) and the Major State Basic Research
Project of China (G1999053902)
#These authors contributed equally to
this work
*Corresponding author: Tel, 86-431-5099768; Fax, 86-431-5681833;
E-mail, [email protected]