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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(9): 623–628 CN 31-1940/Q
Interaction between Plasminogen Activator Inhibitor Type-2 and Pre-mRNA
Processing Factor 8
Jing FAN, Yu-Qing ZHANG, Ping LI, Chang TONG, Li TAN, and Yun-Song ZHU*
Department of Molecular Genetics, Shanghai Medical School of Fudan
University, Key Laboratory of Molecular Medicine, Ministry of Education,
Shanghai 200032, China
Abstract The plasminogen activator inhibitor type-2 (PAI-2) dependent
apoptosis protection is due to the 33 amino acids fragment located between
helix C and D of PAI-2, this fragment may interact with some unknown
intracellular proteins. In this study we used the fragment between helix C and
D of PAI-2 as a bait to perform a yeast two-hybrid screen using a cDNA library
constructed with HeLa cells during apoptosis, and retrieved a clone encoding 94
amino acid residues of C-terminus of pre-mRNA processing factor 8 (PRPF8).
Co-immunoprecipitation experiments confirmed that PAI-2 could interact with
PRPF8 in vivo. PAI-2 could bind PRPF8 C-terminal in both the inside and
outside of nuclear. These results suggested that the interaction between these
two proteins might not be involved in the apoptosis process.
Key words plasminogen activator
inhibitor type-2; pre-mRNA processing factor 8 (PRPF8); yeast two-hybrid system; U5
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Received: June 9,
2004
Accepted: July 29, 2004
This work was supported
by a grant from the National Natural Science Foundation of China (No. 30070412)
*Corresponding
author: Tel, 96-21-54237278; E-mail, [email protected]