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ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(1): 25–31
CN 31-1940/Q
Interaction between a Nanovirus-like Component and the Tobacco
Curly Shoot Virus/Satellite Complex
Pei-Jun WU and Xue-Ping ZHOU*
Institute of Biotechnology, Zhejiang University, Hangzhou 310029,
China
Abstract The biological role of
DNA1, a nanovirus-like component shown to be associated with the begomovirus/satellite
complex, has not yet been identified. Here, we demonstrated that DNA1 of Tobacco
curly shoot virus isolate Y35 (TbCSV-Y35) attenuated leaf-curling symptoms
induced by TbCSV-Y35 or TbCSV-Y35 plus Y35 DNAb in the early stage of
symptom development and induced leaf cluster at a later stage of symptom
development in Nicotiana benthamiana plants. The leaf disc assay
demonstrated that TbCSV-Y35 DNA1 replicated autonomously. Southern blot
analysis revealed that TbCSV-Y35 DNA1 reduced viral DNA accumulation. Viral DNA
accumulation was not reduced when plants were co-inoculated with TbCSV-Y35 DNAb, but the TbCSV-Y35 DNAb level was
dramatically reduced in the presence of TbCSV-Y35 DNA1. To determine whether
the interaction between TbCSV/satellite complex and DNA1 had isolate
specificity, DNA1 of TbCSV isolate Y132 was cloned and sequenced. It was found
to have 75% nucleotide sequence identity with TbCSV-Y35 DNA1. Infectivity tests
showed that TbCSV-Y132 DNA1 had no effect on the symptoms induced by TbCSV-Y35
or TbCSV-Y35 and Y35 DNAb in N. benthamiana plants, although Y132 DNA1 could
replicate in these plants.
Key words Tobacco curly shoot
virus; DNA1; DNAb; symptom; replication; interaction
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Received: September 24, 2004 Accepted: November 18,
2004
This work was supported by the grants from the National Out standing Youth
Foundation (No. 30125032) and the National Natural Science Foundation of China
(No. 30370927)
*Corresponding author: Tel, 86-571-86971680; Fax, 86-571-86971498;
E-mail, [email protected]