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ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(2): 97–106
CN 31-1940/Q
Proteomics-based Identification of Proteins with Altered Expression
Induced by 12-O-Tetradecanoylphorbol 13-acetate in Nasopharyngeal
Carcinoma CNE2 Cells
Pei-Zhou JIANG1,2, Ming GAN3, Hua HUANG1,
Xin-Ming SHEN1, Shuang WANG1, and Kai-Tai YAO1*
1Cancer Research Institute, Southern Medical University, Guangzhou
510515, China;
2Department of Histology and Embryology, Southern Medical University,
Guangzhou 510515, China;
3Department of Parasitology, Zhongshan Medical College, SUN Yat-sen
University, Guangzhou 510080, China
Abstract Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence
in Southern China and South-East Asia. Etiology studies indicate that chemical
carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate
(TPA), are important factors causing NPC development. However, the mechanism of
the TPA effect on NPC remains unclear. In the present study, cells from a
poorly differentiated squamous cell carcinoma NPC cell line, CNE2, were
stimulated by TPA and proteomics technology was carried out to find protein
discrepancies between control and TPA-treated cells. Results revealed that TPA
treatment in CNE2 cells could upregulate the expression of “triosephosphate
isomerase” and “14-3-3 protein sigma” and downregulate the expression of
“reticulocalbin 1 precursor”, “nucleophosmin”, “mitochondrial matrix protein p1
precursor”, and “stathmin”. The changes in the expression of these genes suggested
that TPA induced CNE2 cells to antiproliferation and to apoptosis, which was
confirmed by subsequent apoptosis detection. Therefore, the effects of TPA on
nasopharyngeal carcinoma cells were distinct from the effects on primary
epithelial cells and we suggest reasons for these differences.
Key words 12-O-tetradecanoyl-phorbol-13-acetate; nasopharyngeal
carcinoma; proteomics; apoptosis
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Received: November 1, 2004 Accepted: December 23,
2004
*Corresponding author: Tel/Fax, 86-20-61648225; Fax, 86-20-61648225;
E-mail, [email protected]