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ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(3): 186–191
CN 31-1940/Q
Isolation, Sequence Analysis and Expression Profile of a Novel Swine
Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from
Landrace×Large White Cross-combination
Yong-Gang LIU, Yuan-Zhu XIONG*,
and Chang-Yan DENG
Key Laboratory of Swine Genetics and Breeding, Ministry of
Agriculture, Huazhong Agricultural University, Wuhan 430070, China
Abstract The mRNA differential display
technique was performed to investigate the differences in gene expression in
the Longissimus dorsi muscle tissues from Landrace×Large White
cross-combination. One novel gene that was differentially expressed was
identified using semi-quantitative reverse transcriptase-polymerase chain
reaction (RT-PCR) and its complete cDNA sequence was obtained using the rapid
amplification of cDNA ends (RACE) method. The nucleotide sequence of the gene
is not homologous to any of the known porcine genes. The sequence prediction
analysis revealed that the open reading frame of this gene encodes a protein of
260 amino acids that contains the putative conserved domain of the carbonic
anhydrase, and this protein has high homology with the carbonic anhydrase III
(CA-III) of four species mouse (91%), horse (91%), rat (89%) and human (86%)––so that it can be
defined as swine carbonic anhydrase III. The phylogenetic tree analysis
revealed that the swine CA-III has a closer genetic relationship with the horse
CA-III than with those of mouse, rat and human. The tissue expression analysis
indicated that the swine CA-III gene is generally expressed in most tissues.
Our experiment is the first to establish the primary foundation for further
research on the swine CA-III gene.
Key words mRNA differential
display; rapid amplification of cDNA ends (RACE); novel gene; carbonic
anhydrase III (CA-III)
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Received: October 18, 2004 Accepted: January 22,
2005
This work is supported by a grant from the National Basic Research Program
of China (No. G2000016105).
*Corresponding author: Tel, 86-27-87287390; Fax, 86-27-87394184;
E-mail, [email protected]