https://www.abbs.info
E-mail: [email protected]
ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(3): 192–198
CN 31-1940/Q
Synthesis of Reassortant Infectious Bursal Disease Virus in Chickens
Injected Directly with Infectious Clones from Different Virus Strains
Long LI, Yao-Wei HUANG#, Lian-Sheng WANG, Wang-Jun WAN,
and Lian YU*
Zhejiang Provincial Key Laboratory of Preventive Veterinary
Medicine, Institute of Preventive Veterinary Medicine,
Zhejiang University, Hangzhou 310029, China
Abstract The infectious bursal disease
virus (IBDV), a member of the Birnaviridae family, containing a
bisegmented double-stranded RNA genome, encodes four structural viral proteins,
VP1, VP2, VP3, and VP4, as well as a non-structural protein, VP5. In the
present paper, the segment A from two IBDV strains, field isolate ZJ2000 and
attenuated strain HZ2, were inserted into one NaeI site by site-directed
silent mutagenesis and subcloned into the eukaryotic expression plasmid pCI
under the control of the human cytomegalovirus (hCMV) immediate early enhancer
and promoter to construct the recombinant plasmids pCI-AKZJ2000 and pCI-AKHZ2,
respectively. Each of the two recombinants was combined with another
recombinant pCI plasmid containing the marked segment B of strain HZ2 (pCI-mB),
and injected intramuscularly into non-immunized chickens. Two chimeric IBDV
strains were recovered from the chickens. Two out of eight chickens in each of
two groups showed the bursal histopathological change. The reassortant virus derived from
pCI-AKZJ2000/pCI-mB can infect chicken embryos and shows relatively low
virulence. We have developed a novel virus reverse genetic approach for the
study of IBDV. The results also form the basis for investigating the role of
VP1 in viral replication and pathogenecity.
Key words infectious bursal
disease; infectious bursal disease virus; reverse genetics; reassortant virus;
animal model
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Received: December 29, 2004 Accepted: January 19,
2005
This work was supported by the grants from the National High Technology
Research and Development Program of China (No. 2004BA757C) and the Key Project
of Zhejiang Province (No. 2003C22002)
# Present address: Center for
Molecular Medicine and Infectious Diseases, Virginia Polytechnic Institute and
State University, USA
*Corresponding author: Tel/Fax, 86-571-86971894; E-mail,
[email protected]