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ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(5): 355–361
CN 31-1940/Q
Possible Effect of 30K Proteins in Embryonic Development of Silkworm
Bombyx mori
Bo-Xiong ZHONG1*,
Jian-Ke LI1,2, Jian-Rong LIN3, Jian-She LIANG4,
Song-Kun SU1, Hai-Sheng XU1, Hai-Yan YAN1,
Ping-Bo ZHANG5, and Hiroshi FUJII5
1Laboratory of Molecular Biology of Silkworm and Honeybee, Department
of Bioresource Science, College of Animal Science,
Zhejiang University, Hangzhou 310029, China; 2Institute of Apicultural Research, Chinese Academy of Agricultural
Science, Beijing 100093, China;
3Department of Sericulture, South China Agricultural University,
Guangzhou 510642, China;
4Biochemistry Co. Ltd. of Zhejiang University, Hangzhou 310029,
China;
5Faculty of Agriculture, Kyushu University, Fukuoka 812-81, Japan
Abstract The silkworm Bombyx mori possesses a 30K protein
family of 3×104 Da, the biological functions of which have not
been fully identified. The relationship between the 30K protein family and the
embryonic development of temperature sensitive sex-linked mutant strain of
silkworm was investigated by two
dimensional polyacrylamide gel electrophoresis (2D-PAGE) and Matrix assisted
laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).
The results show that protein spots 1–5 of the 30K protein family, mainly
existing in normal strain, are possibly related to embryonic development. The
early consumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K
proteins named 6G1-30K-3 and 6G1-30K-4 are likely caused by the destruction of
physiological balance in normal embryonic development, which may lead to lower
hatchability of the temperature sensitive strain. The results suggest that
reasonable metabolism of 30K proteins is a prerequisite for the embryo’s normal
development.
Key words silkworm Bombyx mori; 30K
protein; embryonic development; 2D-PAGE; MALDI-TOF MS
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Received: January 5, 2005 Accepted: March 17, 2005
This work was supported by grants from the National Natural Science
Foundation of China (No. 30271004) and the Natural Science Foundation of
Guangdong Province (No. 021022)
*Corresponding author: Tel/Fax, 86-571-86971302; E-mail, [email protected]