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ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(6): 379–385
CN 31-1940/Q
Carboxyfluorescein Diacetate Succinimidyl Ester
Fluorescent Dye for Cell Labeling
Xiao-Qi WANG, Xiu-Mei DUAN, Li-Hua LIU, Yan-Qiu FANG, and Yan TAN*
The Central Laboratory, the First Teaching Hospital of Jilin
University, Changchun 130021, China
Abstract Our objective was to
study the properties of the carboxyfluorescein diacetate succinimidyl ester
(CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First,
we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time.
Second, we determined the optimal concentration of CFDA-SE fluorescent dye for
cell labeling. Third, we tested the toxicity of CFDA-SE fluorescent dye on
labeled cells. Finally, we determined the optimal staining time of CFDA-SE
fluorescent dye for cell labeling. The results show that the optimal
concentration of CFDA-SE fluorescent dye for cell labeling varies according to
different cell types. CFDA-SE fluorescent dye is non-toxic to cells as the cell
death rate caused by CFDA-SE labeling is below 5%. The optimal cell labeling
time was determined to be 8 min of incubation with CFDA-SE fluorescent dye. We
concluded that the advantages of using CFDA-SE fluorescent dye for cell labeling
are as follows: (1) the binding of CFDA-SE fluorescent dye to cells is stable;
(2) CFDA-SE fluorescent dye is not toxic and does not modify the viability of
labeled cells; and (3) CFDA-SE fluorescent dye is a suitable fluorochrome for
cell labeling.
Key words carboxyfluorescein
diacetate succinimidyl ester (CFDA-SE); carboxyfluorescein succinimidyl ester
(CFSE); flow cytometry
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Received: December 13, 2004 Accepted: April 7, 2005
This work was supported by the grants from the National Education
Ministry, State Key Basic Research Program of China (No. 20013145), the Key
Technological Program of the Government Agency of Science and Technology of
Jilin Province (No. 20020403, No. 20040414), the Scientific and Technological
Program of Developing Industry Base of Changchun (04-02GG218)
*Corresponding author: Tel, 86-431-5668196; E-mail,
[email protected]