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ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(6): 386–390
CN 31-1940/Q
Dose-dependent Inhibition of Gynecophoral Canal Protein Gene
Expression in Vitro in the Schistosome (Schistosoma japonicum)
by RNA Interference
Guo-Feng CHENG1, Jiao-Jiao LIN1, Yi SHI2,
You-Xin JIN2*, Zhi-Qiang FU1, Ya-Mei JIN1, Yuan-Cong
ZHOU2, and You-Min CAI1*
1 Shanghai
Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Key
Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232,
China; 2 State Key Laboratory of Molecular Biology, Institute of
Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences, Shanghai 200031, China
Abstract The
gynecophoral canal protein gene SjGCP of Schistosoma japonicum that
is necessary for the pairing between the male and female worms is specifically
expressed in the adult male worm. This protein is widely distributed in the
adult female worm after pairing. Reverse transcription-polymerase chain
reaction (RT-PCR) and immunofluorescence were employed to analyze the
relationship between the RNAi effect and dsRNA dosage in the parasites. The
results revealed that the inhibition of SjGCP expression by siRNA is
dose-dependent. RT-PCR analysis showed that the SjGCP transcript level
was reduced by 75% when 100 nM dsRNA was applied.
Key words RNA interference; gynecophoral canal protein gene; Schistosoma
japonicum; dosage
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Received: January 18, 2005 Accepted: March 17, 2005
This work was supported by the grants from the Shanghai Important
Science and Technology Key Program of China (No. 03DZ19231), the Shanghai
Program for Phosphor Plan of China (No. 04QMX1462) and the National Natural
Science Foundation of China (No. 30270311)
*Corresponding authors:
You-Xin JIN: Tel, 86-21-54921222; Fax, 86-21-54921011; E-mail,
[email protected]
You-Min CAI: Tel,
86-21-54082503; Fax, 86-21-54081818; E-mail, [email protected]