|
Acta
Biochim Biophys Sin 2005,37:440-446
doi:10.1111/j.1745-7270.2005.00061.x
BAF Complex Is Closely Related to and Interacts with NF1/CTF and
RNA Polymerase II in Gene Transcriptional Activation
Li-Hui ZHAO1,2, Xue-Qing BA1, Xiao-Guang WANG3, Xiao-Juan ZHU1, Li WANG1, and Xian-Lu ZENG1*
1 Institute of Genetics and Cytology, Northeast Normal University,
Changchun 130024, China;
2 College of Agronomy, Jilin Agricultural University, Changchun
130118, China;
3 Department of Biology, Changchun Normal College, Changchun 130032,
China
Abstract Brg- or
hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family
of mammalian cells, facilitate transcriptional activity by remodeling
nucleosome structure. Brg1 is the core subunit of Brg-associated factor
complexes. In the present study, we investigated the spatial relationship
between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase II (RNAP II)
upon gene transcriptional activation in vivo by employing immuno-gold
labeling. The data showed that Brg1 was closely co-localized with NF1/CTF and
RNAP II in HeLa cells. Moreover, the co-immunoprecipitation assay further
revealed that Brg1 can be isolated together with NF1/CTF and RNAP II in the
ConA-stimulated, but not the resting, T lymphocyte. The combined results
suggested that BAF complexes can interact with NF1/CTF and RNAP II, and this
interaction is closely dependent on the activation of gene transcription.
Key words BAF complex; NF1/CTF;
RNAP II; gene transcription
|
Dowaload
Download