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Acta
Biochim Biophys Sin 2005,37:463-472
doi:10.1111/j.1745-7270.2005.00063.x
cDNA Array Analysis of the Differential Expression Change in
Virulence-related Genes During the Development of Resistance in Candida
albicans
Zheng XU1#, Yong-Bing CAO1#,
Jun-Dong ZHANG1, Ying-Ying CAO1,
Ping-Hui GAO1, De-Jun WANG1, Xu-Ping
FU2, Kang YING2, Wan-Sheng CHEN1, and Yuan-Ying JIANG1*
1 Department of Pharmacology, School of Pharmacy, Second Military
Medical University, Shanghai 200433, China;
2 State Key Laboratory of Genetic Engineering, Institute of
Genetics, School of Life Science, Fudan University, Shanghai 200433, China
Abstract Candida albicans is the most
frequently isolated fungus in immunocompromised patients associated with
mucosal and deep-tissue infections. To investigate the correlation between
virulence and resistance on a gene expression profile in C. albicans,
we examined the changes in virulence-related genes during the development of
resistance in C. albicans from bone marrow transplant patients using a
constructed cDNA array representing 3096 unigenes. In addition to the genes
known to be associated with azole resistance, 16 virulence-related genes were
identified, whose differential expressions were newly found to be associated
with the resistant phenotype. Differential expressions for these genes were
confirmed by RT-PCR independently. Furthermore, the up-regulation of EFG1,
CPH2, TEC1, CDC24, SAP10, ALS9, SNF1,
SPO72 and BDF1, and the down-regulation of RAD32, IPF3636
and UBI4 resulted in stronger virulence and invasiveness in the
resistant isolates compared with susceptible ones. These findings provide a
link between the expression of virulence genes and development of resistance
during C. albicans infection in bone marrow transplant (BMT)
patients, where C. albicans induces hyphal formation and expression
change in multiple virulence factors.
Key words Candida albicans; cDNA array;
resistance; virulence
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