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Acta
Biochim Biophys Sin 2005,37:473-479
doi:10.1111/j.1745-7270.2005.00063.x
Sequence Analysis and
Structural Prediction of the Severe
Acute Respiratory Syndrome Coronavirus nsp5
Jia-Hai LU#*,
Ding-Mei ZHANG#, Guo-Ling WANG#, Zhong-Min GUO#, Juan
LI#,
Bing-Yan TAN, Li-Ping OU-YANG, Wen-Hua LING, Xin-Bing YU, and Nan-Shan ZHONG1
The Public Health School of
Sun Yat-Sen University, Guangzhou 510080, China;
1 Guangzhou
Institute of Respiratory Diseases, Guangzhou Medical College, Guangzhou
510120, China
Abstract The non-structural proteins
(nsp or replicase proteins) of coronaviruses are relatively conserved
and can be effective targets for drugs. Few studies have been conducted
into the function of the severe acute respiratory syndrome coronavirus
(SARS-CoV) nsp5. In this study, bioinformatics methods were employed
to predict the secondary structure and construct 3-D models of the
SARS-CoV GD strain nsp5. Sequencing and sequential comparison was
performed to analyze the mutation trend of the polymerase nsp5
gene during the epidemic process using a nucleotide-nucleotide basic
local alignment search tool (BLASTN) and a protein-protein basic local
alignment search tool (BLASTP). The results indicated that the nsp5
gene was steady during the epidemic process and the protein was homologous
with other coronavirus nsp5 proteins. The protein encoded by the nsp5
gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide
gel electrophoresis (SDS-PAGE). This study provided the foundation
for further exploration of the protein‘s biological function, and contributed
to the search for anti-SARS-CoV drugs.
Key words severe acute
respiratory syndrome (SARS); severe acute respiratory syndrome coronavirus
(SARS-CoV); non-structural protein (nsp); replicase; secondary structure; 3-D
structure
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