|
Acta
Biochim Biophys Sin 2005,37:480-487
doi:10.1111/j.1745-7270.2005.00062.x
Efficient Differentiation of Embryonic Stem Cells into Neurons in Glial
Cell-conditioned Medium under Attaching Conditions
Hai-Bin TIAN1,2,
Zeng-Liang BAI1, Hong WANG2,
Jian-Quan CHEN2, and Guo-Xiang CHENG2*
1 Institute of Development Biology, School of Life Science, Shandong
University, Jinan 250100, China;
2 shanghai Transgenic Research Center,
Shanghai 201203, China
Abstract Embryonic stem (ES) cells can differentiate into neurons in
vitro, which provides hope for the treatment of some neurodegenerative
diseases through cell transplantation. However, it remains a challenge to
efficiently induce ES cells to differentiate into neurons. Here, we show that
murine ES cells can efficiently differentiate into neurons when cultured in
glial cell-conditioned medium (GCM) under attaching conditions without the
formation of embryoid bodies. In comparison with murine embryonic fibroblast-conditioned
medium, we found that GCM has a positive effect on limiting the generation of
non-neuronal cells, such as astrocytes. In addition, compared with suspension
conditions, attaching conditions delay the differentiation process of ES
cells.
Key words embryonic stem cell; glial
cell-conditioned medium; differentiation; neuron; attaching conditions
|
Dowaload
Download