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Abstract |
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| Vol 48 No. 10: 872-882 |
[PDF] [Full Text] |
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| Decreased expression of GLUT4 in male CG-IUGR rats may play a vital role in their increased susceptibility to diabetes mellitus in adulthood |
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| Chang Duan1,
Min Liu2,
Haiyan Xu1,
Weiwei Tang1,
Jiayun Liu1,
Lamei Hou1 and
Lijuan Li1,*
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1Department of Pathophysiology, Zunyi Medical University, Zunyi 563003, China
2Stomatological Hospital Affiliated to Zunyi Medical University, Zunyi 563003, China
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Abstract Rats with intrauterine growth retardation and catch-up growth (CG-IUGR) after birth show increased susceptibility to diabetes mellitus in adulthood. The expression of glucose transporter type 4 (GLUT4) decreases in female IUGR offspring rats with seminutrient restriction during pregnancy. However, the male CG-IUGR rats also display an increased susceptibility to diabetes mellitus in adulthood. Whether there is another factor, besides GLUT4, in male CG-IUGR rat that mediates their susceptibility to diabetes mellitus? The male IUGR rats with catch-up growth were selected as the research objects. CG-IUGR rats had an increased fasting blood glucose level, and increased serum total cholesterol, triglyceride and free fatty acid levels. Glucose tolerance test and insulin tolerance test showed higher glucose levels and much higher insulin levels after a glucose load in CG-IUGR. The mRNA and protein expressions of IRS-2 in liver tissue, and IRS-1 and GLUT4 in skeletal muscle in CG-IUGR rats were down-regulated, but only the GLUT4 down-regulation displayed strong negative correlations with the decreased glucose tolerance capability by Pearson's analysis. The methylation patterns of CpG islands in the promoter regions of IRS-1, IRS-2 and GLUT4 in CG-IUGR rats varied, which was not significantly correlated with their expressions. The male CG-IUGR rats showed decreased glucose tolerant capability, suggesting increased susceptibility to diabetes mellitus in adulthood. The GLUT4 down-regulation may play a vital role in the development of decreased glucose tolerance in male CG-IUGR rats. The methylation modification of the promoter region of GLUT4 does not appear to be involved in its expression. |
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Keywords CG-IUGR; diabetes mellitus; GLUT4; DNA methylation; metabolism |
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Received 2013-2-25
Accepted 2016-5-28 |
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Funding This work was supported by the grants from the National Natural Science Foundation of China (No. 81260131) and Scientific Research Foundation for Excellent Talents in Guizhou Province (2011-58). |
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* Correspondence address Tel/Fax: +86-851-28609547; E-mail: [email protected] |
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