Article

Acta Biochim Biophys Sin 2005,37:440-446

doi:10.1111/j.1745-7270.2005.00061.x

BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase II in Gene Transcriptional Activation

Li-Hui ZHAO1,2, Xue-Qing BA1, Xiao-Guang WANG3, Xiao-Juan ZHU1, Li WANG1, and Xian-Lu ZENG1*

1 Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;

2 College of Agronomy, Jilin Agricultural University, Changchun 130118, China;

3 Department of Biology, Changchun Normal College, Changchun 130032, China

Abstract Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. In the present study, we investigated the spatial relationship between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase II (RNAP II) upon gene transcriptional activation in vivo by employing immuno-gold labeling. The data showed that Brg1 was closely co-localized with NF1/CTF and RNAP II in HeLa cells. Moreover, the co-immunoprecipitation assay further revealed that Brg1 can be isolated together with NF1/CTF and RNAP II in the ConA-stimulated, but not the resting, T lymphocyte. The combined results suggested that BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.

Key words BAF complex; NF1/CTF; RNAP II; gene transcription

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