Article

Acta Biochim Biophys Sin 2005,37:480-487

doi:10.1111/j.1745-7270.2005.00062.x

Efficient Differentiation of Embryonic Stem Cells into Neurons in Glial Cell-conditioned Medium under Attaching Conditions

Hai-Bin TIAN1,2, Zeng-Liang BAI1, Hong WANG2, Jian-Quan CHEN2, and Guo-Xiang CHENG2*

1 Institute of Development Biology, School of Life Science, Shandong University, Jinan 250100, China;

2 shanghai Transgenic Research Center, Shanghai 201203, China

Abstract Embryonic stem (ES) cells can differentiate into neurons in vitro, which provides hope for the treatment of some neurodegenerative diseases through cell transplantation. However, it remains a challenge to efficiently induce ES cells to differentiate into neurons. Here, we show that murine ES cells can efficiently differentiate into neurons when cultured in glial cell-conditioned medium (GCM) under attaching conditions without the formation of embryoid bodies. In comparison with murine embryonic fibroblast-conditioned medium, we found that GCM has a positive effect on limiting the generation of non-neuronal cells, such as astrocytes. In addition, compared with suspension conditions, attaching conditions delay the differentiation process of ES cells.

Key words embryonic stem cell; glial cell-conditioned medium; differentiation; neuron; attaching conditions

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